6YRM
Transaldolase variant T30A from T. acidophilum in complex with D-fructose 6-phosphate Schiff-base intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 148.600, 172.270, 99.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.027 - 1.700 |
R-factor | 0.1759 |
Rwork | 0.174 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s0c |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.027 | 50.000 | 1.800 |
High resolution limit [Å] | 1.700 | 20.000 | 1.700 |
Rmerge | 0.053 | 0.022 | 2.435 |
Rmeas | 0.057 | 0.025 | 2.630 |
Total number of observations | 943694 | ||
Number of reflections | 139170 | 82 | 21689 |
<I/σ(I)> | 17.22 | 55.42 | 1.13 |
Completeness [%] | 99.2 | 78.8 | 98.9 |
Redundancy | 6.781 | 4.256 | 6.929 |
CC(1/2) | 0.999 | 0.998 | 0.434 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 188 mM ammonium acetate (pH 4.4), 10% (w/v) PEG 6000, and 25% (v/v) glycerol |