6YRB
Crystal structure of the tetramerization domain of the glycoprotein Gn (Andes virus) at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.8 |
| Spacegroup name | I 4 |
| Unit cell lengths | 67.854, 67.854, 121.891 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.699 - 2.351 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26550 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.14rc3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.700 | 37.700 | 2.430 |
| High resolution limit [Å] | 2.350 | 9.100 | 2.350 |
| Rmerge | 0.199 | 0.141 | 1.294 |
| Rmeas | 0.209 | 0.149 | 1.366 |
| Rpim | 0.066 | 0.048 | 0.435 |
| Total number of observations | 114803 | 2055 | 10501 |
| Number of reflections | 11445 | 210 | 1080 |
| <I/σ(I)> | 8.1 | 15.7 | 1.7 |
| Completeness [%] | 99.6 | 99 | 96.4 |
| Redundancy | 10 | 9.8 | 9.7 |
| CC(1/2) | 0.988 | 0.986 | 0.455 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M NaCl, 0.1M Hepes 7.5, 35% MPD |
