6YPR
Human histidine triad nucleotide-binding protein 2 (hHINT2) refined to 1.26 A in H32 space group
This is a non-PDB format compatible entry.
Replaces: 4NJYExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0332 |
Spacegroup name | H 3 2 |
Unit cell lengths | 72.076, 72.076, 103.576 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.043 - 1.260 |
Rwork | 0.117 |
R-free | 0.14270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tw2 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.077 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Phasing software | MOLREP (11.4.06) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.043 | 1.280 |
High resolution limit [Å] | 1.260 | 1.260 |
Rmerge | 0.058 | 0.645 |
Rmeas | 0.073 | 0.815 |
Rpim | 0.043 | 0.492 |
Number of reflections | 28102 | 1352 |
<I/σ(I)> | 12.6 | 2 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 4.8 | 4.5 |
CC(1/2) | 0.998 | 0.685 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 281 | 0.8 M Na/K Tartrate, 0.1 M HEPES 7.5 |