6YPK
Crystal Structure of CK2alpha with GTP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.700, 45.370, 63.490 |
| Unit cell angles | 90.00, 110.98, 90.00 |
Refinement procedure
| Resolution | 22.310 - 1.790 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cvh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.700 | 49.700 | 1.840 |
| High resolution limit [Å] | 1.790 | 8.010 | 1.790 |
| Rmerge | 0.138 | 0.089 | 0.806 |
| Rmeas | 0.165 | 0.107 | 0.970 |
| Rpim | 0.090 | 0.059 | 0.533 |
| Total number of observations | 94177 | 1043 | 6779 |
| Number of reflections | 28931 | 348 | 2102 |
| <I/σ(I)> | 6.5 | 13.1 | 1.5 |
| Completeness [%] | 99.2 | 96.2 | 99 |
| Redundancy | 3.3 | 3 | 3.2 |
| CC(1/2) | 0.982 | 0.976 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 112.5mM Mes, 35% glycerol ethoxylate, 180 mM ammonium acetate |
