6YOQ

Crystal structure of YTHDC1 with compound VVR_DC1_002

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.350, 103.720, 41.870
Unit cell angles	90.00, 106.23, 90.00

Refinement procedure

Resolution	40.200 - 1.300
R-factor	0.1868
Rwork	0.186
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.005
RMSD bond angle	0.820
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.200	1.380
High resolution limit [Å]	1.300	1.300
Number of reflections	78421	12597
<I/σ(I)>	14.32
Completeness [%]	99.5
Redundancy	4.428
CC(1/2)	0.999	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5