6YOO
Structure of SAMM50 LIR bound to GABARAPL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.980, 54.040, 62.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.760 - 1.060 |
R-factor | 0.1349 |
Rwork | 0.134 |
R-free | 0.14750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hoi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.081 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.760 | 1.100 |
High resolution limit [Å] | 1.060 | 1.060 |
Rmerge | 0.060 | 2.500 |
Rpim | 0.010 | 0.790 |
Number of reflections | 825136 | 72954 |
<I/σ(I)> | 17.2 | 1.2 |
Completeness [%] | 99.4 | |
Redundancy | 12.2 | |
CC(1/2) | 1.000 | 0.620 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 24% w/v PEG 6000, 10 mM ZnCl2 and 0.1 M Tris pH 7.5 |