6YO6
Structure of iC3b1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-23 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.87313 |
Spacegroup name | P 1 |
Unit cell lengths | 87.450, 100.600, 136.280 |
Unit cell angles | 94.23, 108.12, 113.81 |
Refinement procedure
Resolution | 47.592 - 6.000 |
R-factor | 0.2434 |
Rwork | 0.241 |
R-free | 0.26620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ehg |
RMSD bond length | 0.035 |
RMSD bond angle | 1.117 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2614) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.592 | 47.592 | 6.160 |
High resolution limit [Å] | 6.000 | 26.830 | 6.000 |
Rmerge | 0.152 | 0.037 | 0.990 |
Rmeas | 0.182 | 0.044 | 1.190 |
Total number of observations | 30364 | ||
Number of reflections | 9685 | 92 | 729 |
<I/σ(I)> | 5.24 | 18.9 | 0.95 |
Completeness [%] | 98.2 | 83.6 | 99 |
Redundancy | 3.135 | 2.913 | 3.111 |
CC(1/2) | 0.993 | 0.996 | 0.562 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293.15 | 96 mM Bis-TRIS Propane pH 8.5, 4 mM Bis-TRIS Propane pH 7, 6.5 % (w/v) PEG 20.000 |