6YN1

Crystal structure of histone chaperone APLF acidic domain bound to the histone H2A-H2B-H3-H4 octamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	105.224, 189.686, 204.324
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.350
R-factor	0.1796
Rwork	0.177
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hio
RMSD bond length	0.009
RMSD bond angle	0.954
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.330
High resolution limit [Å]	2.200	2.200
Rmerge	0.139	3.200
Rmeas	0.151	3.400
Rpim	0.058
Number of reflections	206799	23719
<I/σ(I)>	11.1	0.54
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.990	0.170

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M sodium cacodylate pH6.5, 1.0 M tri-sodium citrate