6YN0

Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 2
Unit cell lengths	63.143, 283.021, 62.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.170 - 2.400
R-factor	0.226
Rwork	0.225
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hla
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.170	2.510
High resolution limit [Å]	2.400	2.400
Rmerge	0.250
Rmeas	0.265	3.810
Number of reflections	25830	6891
<I/σ(I)>	8.1	1.5
Completeness [%]	95.5	85.9
Redundancy	8.5	7.2
CC(1/2)	0.995	0.279

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	(NH4)2SO4 0.1M, sodium Formate 0.3M, Tris-Hcl 0,1M, low molecular weight poly-glutamic acids (PGA-LM) 3% w/v, PEG 550 MME 20% v/v