6YM2

Crystal structure of YTHDC1 with compound ADO_AC_25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.650, 103.210, 42.450
Unit cell angles	90.00, 107.30, 90.00

Refinement procedure

Resolution	40.530 - 1.700
R-factor	0.1995
Rwork	0.197
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.006
RMSD bond angle	0.812
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.540	1.800
High resolution limit [Å]	1.700	1.700
Number of reflections	35644	5703
<I/σ(I)>	11.32
Completeness [%]	99.6
Redundancy	4.57
CC(1/2)	0.999	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5