6YK0
Crystal structure of mouse pyridoxal kinase in complex with ATP-gamma-S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9724 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 278.600, 53.021, 109.851 |
Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
Resolution | 47.160 - 2.900 |
R-factor | 0.2347 |
Rwork | 0.234 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yxt |
RMSD bond length | 0.005 |
RMSD bond angle | 0.731 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.160 | 3.030 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.100 | 0.739 |
Rmeas | 0.083 | 0.631 |
Rpim | 0.068 | 0.488 |
Number of reflections | 36069 | 3532 |
<I/σ(I)> | 9.3 | 1.6 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 3.4 | 3.5 |
CC(1/2) | 0.995 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.18-0.24 M sodium thiocyanate and 18-26% PEG 3350 |