6YJX

Structure of Hen egg-white lysozyme crystallized with PAS polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	P 43 21 2
Unit cell lengths	79.468, 79.468, 37.848
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.110 - 1.200
R-factor	0.1472
Rwork	0.146
R-free	0.17540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3f
RMSD bond length	0.012
RMSD bond angle	1.889
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.110	28.110	1.300
High resolution limit [Å]	1.200	10.000	1.200
Rmerge	0.037	0.025	0.911
Rmeas	0.040	0.027	0.973
Total number of observations	320914
Number of reflections	38399	80	8070
<I/σ(I)>	26.02	66.32	2.44
Completeness [%]	99.8	88.9	99.8
Redundancy	8.357	5.7	8.178
CC(1/2)	1.000	1.000	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	12.5 % (w/v) PA200 0.1 M NaOAc 0.2 M NaCl