6YJP
Crystal structure of a complex between glycosylated NKp30 and its deglycosylated tumour ligand B7-H6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.044, 86.462, 111.299 |
| Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
| Resolution | 48.950 - 3.100 |
| R-factor | 0.2748 |
| Rwork | 0.272 |
| R-free | 0.32210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pv6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.590 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0258 (LORESTR pipeline))) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 3.290 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.104 | 0.971 |
| Rmeas | 0.134 | |
| Rpim | 0.081 | 0.804 |
| Number of reflections | 27102 | 3968 |
| <I/σ(I)> | 5.7 | 0.8 |
| Completeness [%] | 95.0 | 87 |
| Redundancy | 3.7 | 3.1 |
| CC(1/2) | 0.995 | 0.645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 294 | 0.1 M sodium citrate pH 6.7, 11.7 % PEG 6000, 10 mg/ml mixture of both proteins, micro-seeded with crushed needle-shaped crystals |
