6YIP
Structure of MKLP2 coiled coil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2018-08-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96862 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.240, 32.925, 159.735 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.250 - 1.430 |
R-factor | 0.2239 |
Rwork | 0.219 |
R-free | 0.32040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lef |
RMSD bond length | 0.013 |
RMSD bond angle | 1.423 |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.870 | 1.660 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.162 | 1.486 |
Number of reflections | 11058 | 554 |
<I/σ(I)> | 9.2 | 1.8 |
Completeness [%] | 87.9 | |
Redundancy | 13.7 | |
CC(1/2) | 0.997 | 0.854 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 291 | 20% (w/v) PEG 4,000, 20 % (v/v) 2-propanol, 100 mM sodium citrate, pH 5.6. |