6YIP

Structure of MKLP2 coiled coil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	110
Detector technology	PIXEL
Collection date	2018-08-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96862
Spacegroup name	P 21 21 21
Unit cell lengths	30.240, 32.925, 159.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.250 - 1.430
R-factor	0.2239
Rwork	0.219
R-free	0.32040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lef
RMSD bond length	0.013
RMSD bond angle	1.423
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.870	1.660
High resolution limit [Å]	1.430	1.430
Rmerge	0.162	1.486
Number of reflections	11058	554
<I/σ(I)>	9.2	1.8
Completeness [%]	87.9
Redundancy	13.7
CC(1/2)	0.997	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	291	20% (w/v) PEG 4,000, 20 % (v/v) 2-propanol, 100 mM sodium citrate, pH 5.6.