6YGC

Crystal structure of the NatC complex bound to Arl3 peptide and CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	48.303, 139.724, 166.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.397 - 2.994
R-factor	0.1986
Rwork	0.196
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yga
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.397	46.397	3.170
High resolution limit [Å]	2.990	8.830	2.990
Rmerge	0.166	0.031	0.884
Rmeas	0.191	0.037	1.013
Total number of observations	94075
Number of reflections	23309	987	3667
<I/σ(I)>	8.42	33.21	1.6
Completeness [%]	98.7	95.4	98.7
Redundancy	4.036	3.616	4.145
CC(1/2)	0.991	0.998	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	16% PEG 4000, 150 mM ammonium iodide and 100 mM sodium citrate, pH 6.2