6YFZ
Crystal structure of MKK7 (MAP2K7), apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.02823 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.460, 74.451, 81.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.410 - 1.900 |
R-factor | 0.1944 |
Rwork | 0.192 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dyl |
RMSD bond length | 0.016 |
RMSD bond angle | 1.678 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.410 | 30.408 | 2.000 |
High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
Rmerge | 0.103 | 0.044 | 0.843 |
Rmeas | 0.113 | 0.048 | 0.922 |
Rpim | 0.045 | 0.020 | 0.367 |
Number of reflections | 28220 | 998 | 4047 |
<I/σ(I)> | 9.2 | 10.8 | 0.9 |
Completeness [%] | 99.6 | 98.6 | 99.6 |
Redundancy | 6.1 | 5.6 | 6.1 |
CC(1/2) | 0.996 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277.15 | 19% PEG3350, 0.15 M ammonium acetate, 0.1 M tris, pH 7.8 |