6YEV

Crystal structure of MsrA C206 and Trx C35S complex from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2019-09-27
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 32 2 1
Unit cell lengths	139.910, 139.910, 218.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.710 - 2.940
R-factor	0.2302
Rwork	0.229
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2trx 1ff3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17rc5_3630)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.710	3.010
High resolution limit [Å]	2.940	2.940
Rmeas	0.550
Number of reflections	53069	5325
<I/σ(I)>	0.47
Completeness [%]	99.8
Redundancy	39.8
CC(1/2)		0.112

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	0.1M BIS-TRIS propane pH 6.5, 0.2M trisodium citrate, 20% PEG 3350