6YEK

Crystal structure of human NEMO apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 43 2 2
Unit cell lengths	85.760, 85.760, 99.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.760 - 3.200
R-factor	0.2954
Rwork	0.291
R-free	0.33590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f89
RMSD bond length	0.014
RMSD bond angle	1.859
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.790	3.320
High resolution limit [Å]	3.200	3.200
Number of reflections	6469	638
<I/σ(I)>	35.8	8.2
Completeness [%]	99.7	100
Redundancy	6.2	6.5
CC(1/2)	0.999	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	45% (v/v) ethylene glycol 50mM acetate (pH 4.0)