6YEH

Arabidopsis thaliana glutamate dehydrogenase isoform 1 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8266
Spacegroup name	P 21 21 21
Unit cell lengths	93.570, 99.169, 318.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.770 - 2.590
R-factor	0.1835
Rwork	0.183
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AtGDH1-NAD
RMSD bond length	0.006
RMSD bond angle	0.849
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.000	88.900	2.760
High resolution limit [Å]	2.590	7.950	2.590
Rmerge	0.190	0.043	1.070
Rpim	0.074	0.025	0.390
Number of reflections	69315	3467	3405
<I/σ(I)>	9.6	26.4	2.3
Completeness [%]	88.9	99.3	42.2
Redundancy	7.4	6.3	8.5
CC(1/2)	0.995	0.998	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	20% w/v PEG 4000, 100 mM Tris-HCl pH 7.5, 33% (v/v) MPD