6YCQ
Crystal structure of the DNA binding domain of Arabidopsis thaliana Auxin Response Factor 1 (AtARF1) in complex with High Affinity DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.304, 102.784, 127.039 |
Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
Resolution | 47.570 - 1.650 |
R-factor | 0.173 |
Rwork | 0.172 |
R-free | 0.19910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ldx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.963 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.580 | 1.680 |
High resolution limit [Å] | 1.650 | 1.651 |
Number of reflections | 75146 | 82 |
<I/σ(I)> | 15.9 | |
Completeness [%] | 88.1 | |
Redundancy | 3.4 | |
CC(1/2) | 0.999 | 0.512 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | B10 condition of the Morpheus HT screen: 0.09 M (0.3M Sodium fluoride, 0.3M Sodium bromide, 0.3M Sodium iodide); 0.1 M (Tris (base), BICINE pH8.5); 20% v/v Ethylene glycol; 10 % w/v PEG 8000 |