6YCA

Crystal structure of Eis1 from Mycobacterium abscessus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-01
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	118.390, 150.690, 166.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.270 - 2.900
R-factor	0.2097
Rwork	0.208
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rft
RMSD bond length	0.004
RMSD bond angle	0.722
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmeas	0.190	0.780
Number of reflections	65973	6337
<I/σ(I)>	9.3
Completeness [%]	98.8	99.7
Redundancy	4.5
CC(1/2)	0.980	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.8 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5 and 1% PEG 3350