6YC0
Crystal structure of the steady-state-SMX activated state of the light-driven sodium pump KR2 in the pentameric form at room temperature, pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2019-07-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 135.210, 240.340, 138.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.700 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xyt |
| RMSD bond length | 0.001 |
| RMSD bond angle | 1.075 |
| Data reduction software | CrystFEL |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.190 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 77967 | 7691 |
| <I/σ(I)> | 11.3 | 0.72 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 894.5 | 640.6 |
| CC(1/2) | 0.988 | 0.488 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 293 | 1.2 M NaMal pH 8.0 |
