6YAX
Crystal structure of CD32b (Fc Gamma Receptor IIb) in complex with Human IgG1 Fab fragment (5C05)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-24 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.97 |
Spacegroup name | P 43 |
Unit cell lengths | 95.728, 95.728, 186.038 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.860 - 2.800 |
Rwork | 0.230 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5occ |
RMSD bond length | 0.004 |
RMSD bond angle | 1.383 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.860 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 41024 | 3003 |
<I/σ(I)> | 9.1 | |
Completeness [%] | 99.3 | |
Redundancy | 7.2 | |
CC(1/2) | 0.995 | 0.491 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M 1,6-hexanediol, 0.2 M 1-butanol, 0.2 M 1,2-propanediol, 0.2 M 2- propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol, 1.0 M tris (base), BICINE, 40% v/v PEG 500 MME, 20% w/v PEG 20000 |