6YAU
CRYSTAL STRUCTURE OF ASGPR 1 IN COMPLEX WITH GN-A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999910 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.750, 32.530, 39.650 |
| Unit cell angles | 90.00, 93.82, 90.00 |
Refinement procedure
| Resolution | 57.250 - 1.397 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dv8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.250 | 1.558 |
| High resolution limit [Å] | 1.397 | 1.397 |
| Rmerge | 0.078 | 0.912 |
| Number of reflections | 16299 | 815 |
| <I/σ(I)> | 11.1 | 1.4 |
| Completeness [%] | 55.7 | 10.1 |
| Redundancy | 6.3 | 5.1 |
| CC(1/2) | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Protein Buffer: 20 mM Tris-HCl (pH 7.4), 25 mM CaCl2. Reservoir: 100 mM Tris-Hcl (pH 7.5), 28% PEG4000 |
