6Y8P
Crystal structure of SNAP-tag labeled with a benzyl-tetramethylrhodamine fluorophore
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 65.515, 65.515, 97.067 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.980 - 2.300 |
R-factor | 0.2374 |
Rwork | 0.236 |
R-free | 0.27670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l00 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.980 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 11154 | 1087 |
<I/σ(I)> | 15 | |
Completeness [%] | 99.9 | |
Redundancy | 10.7 | |
CC(1/2) | 0.999 | 0.193 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 100 mM Na HEPES pH 7.5 25% PEG 8000 10 mg/mL protein 100 nl:100 nL |