6Y7N

The crystal structure of the eight-bladed symmetrical designer protein Tako8 in the presence of tellurotungstic Anderson-Evans (TEW)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97951
Spacegroup name	P 1 21 1
Unit cell lengths	73.586, 60.929, 73.613
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.930 - 1.600
R-factor	0.2188
Rwork	0.217
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g6m
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.940	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.025	0.074
Rmeas	0.033	0.103
Rpim	0.023	0.070
Number of reflections	85806	4215
<I/σ(I)>	21.4	8.3
Completeness [%]	99.8
Redundancy	3.3
CC(1/2)	0.997	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	293	0.1M sodium acetate, 3.1M sodium chloride