Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

6G6M

Crystal structure of the computationally designed Tako8 protein in P42212

Summary for 6G6M
Entry DOI10.2210/pdb6g6m/pdb
DescriptorTako8, SULFATE ION (3 entities in total)
Functional Keywordsartificial protein, de novo protein, wd40 proteins
Biological sourcesynthetic construct
Total number of polymer chains1
Total formula weight35408.48
Authors
Noguchi, H.,Addy, C.,Simoncini, D.,Van Meervelt, L.,Schiex, T.,Zhang, K.Y.J.,Tame, J.R.H.,Voet, A.R.D. (deposition date: 2018-04-01, release date: 2018-11-28, Last modification date: 2024-05-08)
Primary citationNoguchi, H.,Addy, C.,Simoncini, D.,Wouters, S.,Mylemans, B.,Van Meervelt, L.,Schiex, T.,Zhang, K.Y.J.,Tame, J.R.H.,Voet, A.R.D.
Computational design of symmetrical eight-bladed beta-propeller proteins.
IUCrJ, 6:46-55, 2019
Cited by
PubMed Abstract: β-Propeller proteins form one of the largest families of protein structures, with a pseudo-symmetrical fold made up of subdomains called blades. They are not only abundant but are also involved in a wide variety of cellular processes, often by acting as a platform for the assembly of protein complexes. WD40 proteins are a subfamily of propeller proteins with no intrinsic enzymatic activity, but their stable, modular architecture and versatile surface have allowed evolution to adapt them to many vital roles. By computationally reverse-engineering the duplication, fusion and diversification events in the evolutionary history of a WD40 protein, a perfectly symmetrical homologue called Tako8 was made. If two or four blades of Tako8 are expressed as single polypeptides, they do not self-assemble to complete the eight-bladed architecture, which may be owing to the closely spaced negative charges inside the ring. A different computational approach was employed to redesign Tako8 to create Ika8, a fourfold-symmetrical protein in which neighbouring blades carry compensating charges. Ika2 and Ika4, carrying two or four blades per subunit, respectively, were found to assemble spontaneously into a complete eight-bladed ring in solution. These artificial eight-bladed rings may find applications in bionanotechnology and as models to study the folding and evolution of WD40 proteins.
PubMed: 30713702
DOI: 10.1107/S205225251801480X
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.7 Å)
Structure validation

227561

PDB entries from 2024-11-20

PDB statisticsPDBj update infoContact PDBjnumon