6Y6D

Tubulin-7-Aminonoscapine complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	104.360, 157.150, 180.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.340 - 2.200
R-factor	0.1858
Rwork	0.184
R-free	0.22380
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4i4t
RMSD bond length	0.002
RMSD bond angle	0.518
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.340	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.138	3.394
Rmeas	0.144	3.529
Rpim	0.039	0.961
Number of reflections	150529	14882
<I/σ(I)>	14.42	0.74
Completeness [%]	100.0	99.95
Redundancy	13.4	13.2
CC(1/2)	0.999	0.259

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	5% PEG 4K, 11% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole, pH 6.5