6Y64

Structure of Sheep Polyomavirus VP1 in complex with 6'-Sialyllactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	129.620, 81.260, 146.660
Unit cell angles	90.00, 115.52, 90.00

Refinement procedure

Resolution	47.470 - 1.600
R-factor	0.15197
Rwork	0.152
R-free	0.18218
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fmg
RMSD bond length	0.009
RMSD bond angle	1.510
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.470	1.657
High resolution limit [Å]	1.600	1.600
Rmeas	0.071	0.727
Number of reflections	350203	34182
<I/σ(I)>	17.78	2.94
Completeness [%]	96.8
Redundancy	6.8
CC(1/2)	0.999	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Magnesium chloride, PEG 800, TRIS