6Y60

Structure of Human Polyomavirus 12 VP1 in complex with 3'-Sialyllactosamine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2013-06-27
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	83.322, 141.724, 251.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.370 - 1.798
Rwork	0.203
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fmg
RMSD bond length	0.009
RMSD bond angle	1.629
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.370	1.910
High resolution limit [Å]	1.798	1.798
Rmeas	0.125	1.770
Number of reflections	274251	42872
<I/σ(I)>	12.21	1.34
Completeness [%]	99.4
Redundancy	7.04
CC(1/2)	0.999	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Ammonium acetate, Bis-Tris, 2-methyl-2,4-pentanediol