6Y5Y
Structure of New Jersey Polyomavirus VP1 in complex with 3'-Sialyllactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2014-09-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.381, 151.082, 130.617 |
Unit cell angles | 90.00, 106.56, 90.00 |
Refinement procedure
Resolution | 48.200 - 1.800 |
R-factor | 0.1668 |
Rwork | 0.166 |
R-free | 0.20640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fmg |
RMSD bond length | 0.006 |
RMSD bond angle | 0.852 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 1.865 |
High resolution limit [Å] | 1.800 | 1.801 |
Rmeas | 0.156 | 1.176 |
Number of reflections | 295112 | 29218 |
<I/σ(I)> | 9.79 | 1.39 |
Completeness [%] | 99.8 | |
Redundancy | 5.7 | |
CC(1/2) | 0.996 | 0.603 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Succinic acid, PEG 3350 |