6Y5C
The crystal structure of glycogen phosphorylase in complex with 52
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION SUPERNOVA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2018-05-30 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 127.957, 127.957, 115.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.680 - 2.400 |
| R-factor | 0.17035 |
| Rwork | 0.168 |
| R-free | 0.21117 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.941 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.680 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.101 | 0.623 |
| Number of reflections | 34314 | 3151 |
| <I/σ(I)> | 10.7 | 1.4 |
| Completeness [%] | 91.2 | 80.2 |
| Redundancy | 4.8 | 3.4 |
| CC(1/2) | 0.988 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer, pH 6.7 |
