6Y4Z

The crystal structure of human MACROD2 in space group P43212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9762
Spacegroup name	P 43 21 2
Unit cell lengths	95.360, 95.360, 261.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.730 - 1.900
R-factor	0.2018
Rwork	0.201
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iqy
RMSD bond length	0.009
RMSD bond angle	1.563
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.730	47.730	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.081	0.024	1.650
Rmeas	0.085	0.025	1.729
Total number of observations	1280920
Number of reflections	95967	1244	7009
<I/σ(I)>	18.92	77.14	1.25
Completeness [%]	100.0	99	100
Redundancy	13.348	12.116	11.395
CC(1/2)	1.000	0.999	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	0.2 M Ammonium tartarate dibasic pH 6.7, 20% PEG 3350