6Y4L

Crystal structure of human ER membrane protein complex subunits EMC2 and EMC9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	52.577, 84.939, 122.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.700 - 2.200
R-factor	0.2064
Rwork	0.203
R-free	0.24990
Structure solution method	SAD
Starting model (for MR)	XXXX
RMSD bond length	0.007
RMSD bond angle	0.844
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.700	2.780	2.270
High resolution limit [Å]	2.200	2.650	2.200
Number of reflections	28668	2109	2433
<I/σ(I)>	17.6	1.8	1.9
Completeness [%]	100.0
Redundancy	51.9
CC(1/2)	1.000	0.817	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	293.15	34% PEG4000, 0.1 M Tris pH 8.3, and 0.2 M lithium sulfate (For I03 native dataset)
1	VAPOR DIFFUSION, HANGING DROP	8.3	293.15	34% PEG4000, 0.1 M Tris pH 8.3, and 0.2 M lithium sulfate (For I03 native dataset)