6Y34
Streptavidin wildtype with a biotC5-1 cofactor - an artificial iron hydroxylase
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 57.941, 57.941, 183.822 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.998 - 1.307 |
Rwork | 0.180 |
R-free | 0.19780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pk2 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.235 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.998 | 1.330 |
High resolution limit [Å] | 1.307 | 1.307 |
Rmerge | 0.070 | 2.702 |
Number of reflections | 38242 | 1785 |
<I/σ(I)> | 26.2 | |
Completeness [%] | 99.3 | 95.4 |
Redundancy | 25.4 | 22.9 |
CC(1/2) | 1.000 | 0.552 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 2 M (NH4)2SO4, 0.1 M Na-Acetate, pH 4 |