6Y30
NG domain of human SRP54 T115A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.795, 53.903, 91.366 |
| Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
| Resolution | 53.257 - 2.650 |
| R-factor | 0.278 |
| Rwork | 0.276 |
| R-free | 0.30860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6y2z |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.459 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.260 | 2.750 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rpim | 0.120 | 0.590 |
| Number of reflections | 22749 | 2164 |
| <I/σ(I)> | 7.51 | |
| Completeness [%] | 95.9 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 1M Potassium Citrate |
