6Y2H
The crystal structure of human chloride intracellular channel protein 5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.990, 93.960, 109.580 |
| Unit cell angles | 90.00, 124.13, 90.00 |
Refinement procedure
| Resolution | 48.005 - 2.154 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ahe |
| Data reduction software | XSCALE |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.005 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.091 | 1.520 |
| Number of reflections | 65103 | 12363 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 97.1 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 0.1M NaSCN, 17%PEG 3350, 7% MEG |
