6Y20

Crystal structure of Protein Scalloped (222-440) bound to Protein Vestigial (298-337)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	48.230, 62.039, 156.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.000 - 1.849
R-factor	0.1894
Rwork	0.188
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6q36
RMSD bond length	0.008
RMSD bond angle	0.990
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.11.7 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.444	2.013
High resolution limit [Å]	1.851	1.851
Number of reflections	30608	1531
<I/σ(I)>	9.337	1.6
Completeness [%]	74.6	16.9
Redundancy	16.7
CC(1/2)	0.994	0.619

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M Magnesium chloride, 0.1 M Bis-Tris pH 6.5, 25% PEG3350