6Y1U

Mycobacterium tuberculosis FtsZ-GDP in complex with 4-hydroxycoumarin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-04-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 65
Unit cell lengths	88.130, 88.130, 176.783
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.320 - 1.680
R-factor	0.1698
Rwork	0.168
R-free	0.19660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rq7
RMSD bond length	0.012
RMSD bond angle	1.216
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.323	1.740
High resolution limit [Å]	1.680	1.680
Number of reflections	88000	8733
<I/σ(I)>	17.5
Completeness [%]	100.0
Redundancy	8
CC(1/2)	0.999	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291.15	27.5% PEG4000, 0.4 M ammonium acetate and 0.1 M Na citrate, soaked with 37.5 mM of inhibitor