6Y0A
CRYSTAL STRUCTURE OF CDK8-CycC IN COMPLEX WITH BI00690300
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99988 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.598, 71.120, 169.785 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 84.890 - 2.190 |
R-factor | 0.20161 |
Rwork | 0.201 |
R-free | 0.22679 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f6s |
RMSD bond length | 0.008 |
RMSD bond angle | 1.242 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.890 | 2.440 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.066 | 0.440 |
Number of reflections | 43086 | 43086 |
<I/σ(I)> | 13.48 | |
Completeness [%] | 96.1 | 96.9 |
Redundancy | 2.8 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 20% (wt/vol) polyethylene glycol 3350 0.2 M sodium formate |