6XZZ
Crystal structure of the BCL6 BTB domain in complex with the NCoR1 BBD2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 67.014, 67.014, 152.016 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.220 - 1.390 |
| R-factor | 0.1857 |
| Rwork | 0.185 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r28 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.795 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.220 | 1.410 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rpim | 0.038 | |
| Number of reflections | 41626 | 2036 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 18.3 | |
| CC(1/2) | 0.999 | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 13.4%(v/v) PEG 400, 0.335M K2HPO4 / NaH2PO4 pH 7.5 |
