6XZZ
Crystal structure of the BCL6 BTB domain in complex with the NCoR1 BBD2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 67.014, 67.014, 152.016 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.220 - 1.390 |
R-factor | 0.1857 |
Rwork | 0.185 |
R-free | 0.20570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r28 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.795 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.220 | 1.410 |
High resolution limit [Å] | 1.390 | 1.390 |
Rpim | 0.038 | |
Number of reflections | 41626 | 2036 |
<I/σ(I)> | 13.2 | |
Completeness [%] | 100.0 | 99.7 |
Redundancy | 18.3 | |
CC(1/2) | 0.999 | 0.524 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 13.4%(v/v) PEG 400, 0.335M K2HPO4 / NaH2PO4 pH 7.5 |