6XZW

Crystal structure of the meningococcal vaccine antigen fHbp in complex with a cross-reactive human Fab.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.966
Spacegroup name	P 1 21 1
Unit cell lengths	47.340, 90.400, 97.940
Unit cell angles	90.00, 98.82, 90.00

Refinement procedure

Resolution	46.780 - 2.400
R-factor	0.2
Rwork	0.200
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kvd 5tlk
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.780	2.480
High resolution limit [Å]	2.400	2.400
Number of reflections	29802	3202
<I/σ(I)>	16.5
Completeness [%]	93.0
Redundancy	3.4
CC(1/2)	0.990	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M HEPES, 20% w/v jeff ED-2001, pH 6.5