6XXO
Crystal structure of NB8, a nanobody targeting prostate specific membrane antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9677 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 55.945, 68.857, 75.647 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.520 - 1.500 |
R-factor | 0.1634 |
Rwork | 0.162 |
R-free | 0.18320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m7q |
RMSD bond length | 0.007 |
RMSD bond angle | 0.943 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.520 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.028 | |
Rmeas | 0.117 | |
Number of reflections | 23283 | 3733 |
<I/σ(I)> | 28.37 | |
Completeness [%] | 97.9 | |
Redundancy | 3.9 | |
CC(1/2) | 0.999 | 0.988 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M tri-Na Citrate pH 3.5, 3M NaCl |