6XXI
Crystal Structure of Human Deoxyhypusine Synthase in complex with NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 104.819, 104.819, 160.499 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.091 - 1.679 |
R-factor | 0.1541 |
Rwork | 0.154 |
R-free | 0.16620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dhs |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (v.1.17.1) |
Refinement software | PHENIX (v.1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.091 | 46.091 | 1.780 |
High resolution limit [Å] | 1.679 | 5.010 | 1.680 |
Rmerge | 0.126 | 0.072 | 2.021 |
Rmeas | 0.135 | 0.077 | 2.153 |
Number of reflections | 116359 | 4631 | 18588 |
<I/σ(I)> | 8.88 | 28.64 | 0.92 |
Completeness [%] | 99.7 | 98.9 | 99.4 |
Redundancy | 8.419 | 8.085 | 8.556 |
CC(1/2) | 0.996 | 0.994 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.025-0.125 mM carboxylic acid mix, 30-60% precipitant mix (MPD, PEG 1000, PEG 3350), 100 mM Tris-Bicine pH = 8.5 |