6XX0
Crystal structure of NEMO in complex with Ubv-LIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2020-01-25 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.323, 80.773, 84.929 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.420 - 2.600 |
R-factor | 0.2437 |
Rwork | 0.238 |
R-free | 0.29060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f89 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.309 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.990 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 101221 | 2213 |
<I/σ(I)> | 10.7 | |
Completeness [%] | 100.0 | |
Redundancy | 6.6 | |
CC(1/2) | 0.997 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 22.5% polyethylene glycol 3350, 0.1M Magnesium chloride, 0.1M Try-HCl, pH 7.0 |