6XWG

Crystal Structure of the Human RXR/RAR DNA-Binding Domain Heterodimer Bound to the Human RARb2 DR5 Response Element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.006
Spacegroup name	P 43 21 2
Unit cell lengths	56.714, 56.714, 238.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.510 - 2.400
R-factor	0.192
Rwork	0.191
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cn5
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.510	26.510	2.550
High resolution limit [Å]	2.400	7.160	2.400
Rmerge	0.111	0.049	5.955
Rmeas	0.118	0.052	6.294
Total number of observations	156137
Number of reflections	16005	720	2468
<I/σ(I)>	9.77	37.91	0.29
Completeness [%]	99.2	100	97.8
Redundancy	9.756	10.004	8.814
CC(1/2)	0.999	0.999	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% PEG 3350, 0.2 M Li2SO4, 0.1 M bis-tris pH 6.5