6XVY
Human myelin protein P2 mutant R88Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-24 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.880, 64.880, 264.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.1611 |
| Rwork | 0.159 |
| R-free | 0.19910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wut |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.085 | 0.841 |
| Number of reflections | 53492 | 3745 |
| <I/σ(I)> | 19.8 | 2 |
| Completeness [%] | 99.4 | 96.9 |
| Redundancy | 7.3 | 5.9 |
| CC(1/2) | 0.999 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 40% PEG 1000, 0.1 M citrate pH 5.0 |
