6XUS
RNA dodecamer with a 6-hydrazino-2-aminopurine modified base
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 41.120, 35.140, 31.870 |
Unit cell angles | 90.00, 129.43, 90.00 |
Refinement procedure
Resolution | 24.620 - 1.100 |
R-factor | 0.1542 |
Rwork | 0.153 |
R-free | 0.17370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q1r |
RMSD bond length | 0.008 |
RMSD bond angle | 1.808 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.070 |
High resolution limit [Å] | 1.040 | 1.040 |
Rmeas | 0.050 | 261.000 |
Number of reflections | 106381 | 1200 |
<I/σ(I)> | 13.32 | 0.29 |
Completeness [%] | 99.1 | 98.6 |
Redundancy | 6.3 | |
CC(1/2) | 1.000 | 0.148 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | MPD, Na Cacodylate, spermine, NaCl, MgCl2 |