6XUR
RNA dodecamer with a 6-hydrazino-2-aminopurine modified base
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 41.420, 35.030, 32.030 |
| Unit cell angles | 90.00, 129.72, 90.00 |
Refinement procedure
| Resolution | 24.640 - 1.100 |
| R-factor | 0.1551 |
| Rwork | 0.154 |
| R-free | 0.18030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q1r |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.978 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.030 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Number of reflections | 113052 | 3237 |
| <I/σ(I)> | 9.66 | |
| Completeness [%] | 97.1 | 76.1 |
| Redundancy | 6 | |
| CC(1/2) | 0.999 | 0.006 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | MPD, sodium cacodylate, spermine tetrahydrochloride, NaCl, MgCl2 |
