6XU0

Archaeoglobus fulgidus Argonaute protein with DNA oligoduplex 5'-pATCGTGGCCACGAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97630
Spacegroup name	P 1
Unit cell lengths	51.909, 61.205, 103.092
Unit cell angles	98.32, 104.96, 100.62

Refinement procedure

Resolution	46.439 - 1.900
R-factor	0.1849
Rwork	0.180
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1ytu
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.785	48.785	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge		0.038	0.638
Rmeas	0.079	0.049	0.894
Rpim	0.040	0.025	0.449
Total number of observations		10888	48635
Number of reflections	85105	2769	12355
<I/σ(I)>	8.3	21.7	1.7
Completeness [%]	91.5	94	90.9
Redundancy	3.9	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	280	Na-Cacodylate pH 6.5 0.05 M, KCl_0.04 M, MgCl2 0.01 M, PEG3350 11% (w/v)